IpheshelweshiIngabe i-molecule edlala indima ebalulekile ekuphendukeni kwamakhemikhali amaningi futhi isetshenziswa ezinhlobonhlobo zezicelo zezimboni. Ngakho-ke, kubalulekile ukuba nendlela ethembekile yokuthola i-phenol kumasampula ahlukahlukene. Kulesi sihloko, sizohlola amasu ahlukahlukene ukuthola ukuthi i-Phenol, izinzuzo zawo kanye nobunzima babo, kanye nokubaluleka kokukhonjwa kwezifo ekuphileni kwansuku zonke nasembonini.

1. I-chromatography yegesi (GC)

I-Gher Chromatography iyindlela yokuhlaziya esetshenziswa kabanzi yokuhlonza i-phenol. Ngale ndlela, isampula ifakwa kwikholamu egcwele isigaba esimile. Isigaba seselula bese sigeleza kukholamu, sihlukanisa izingxenye ngazinye zesampula. Ukwahlukana kususelwa ku-solubility ehlobene nezakhi ezisezigabeni ezimile neziselula.

Izinzuzo: I-GC izwela kakhulu, icacisiwe, futhi ngokushesha. Ingabona ukugxila okuphansi kwe-phenol.

Okubi: I-GC idinga abasebenzi abaqeqeshiwe kakhulu kanye nemishini ebizayo, okwenza kungakulungele ukuhlolwa kwensimu.

2. I-chromatography ye-liquid (LC)

I-Chromatography e-Liquid iyefana ne-chromatography yegesi, kepha isigaba seminyaka sigcwele ikholomu esikhundleni sokuhlanganiswa kokusekelwa okumile. I-LC isetshenziselwa ngokuvamile ukuhlukanisa ama-molecule amakhulu, njengamaprotheni nama-peptides.

Izinzuzo: I-LC inokusebenza kahle okuphezulu futhi ingaphatha ama-molecule amakhulu.

Okubi: I-LC ayizwela kakhulu kune-GC futhi idinga isikhathi esithe xaxa ukuthola imiphumela.

3. Spectroscopy

I-Spectroscopy yindlela engabhubhisayo ehlanganisa ukulinganisa ukumuncwa noma ukuphuma kwemisebe ngama-athomu noma ama-molecule. Endabeni ye-phenol, infrared spectroscopy kanye ne-magnetic kazibuthe yenuzi (NMR) i-spectroscopy ivame ukusetshenziswa. I-infrared spectroscopy ilinganisa ukumuncwa kwemisebe ye-infrared ngama-molecule, kuyilapho i-nmr spectroscopy ilinganisa ukumuncwa kwemisebe ye-radiofrequency ama-athomu.

Izinzuzo: I-Spectroscopy iqondile kakhulu futhi inganikeza imininingwane eningiliziwe mayelana nokwakheka kwama-molecule.

Okubi: I-spectroscopy ivame ukudinga imishini ebizayo futhi ingadla isikhathi.

4. Izindlela zeColotimetric

Izindlela zeColorimetric zibandakanya ukuphendula isampula nge-realent ukukhiqiza umkhiqizo onemibala ongalinganiswa ngokubuka izinto ezibonakalayo. Indlela eyodwa evamile ye-colorimetric yokuhlonza i-phenol ifaka ukuphendula isampula nge-4-aminaantipyrane phambi kwe-realent ehlanganisiwe ukukhiqiza umkhiqizo onemibala ebomvu ukuze ukhiqize umkhiqizo onombala obomvu. Ukuqina kombala kulingana ngqo nokuhlushwa kwesampula.

Izinzuzo: Izindlela zeColorimetric zilula, akubizi, futhi zingasetshenziselwa ukuhlolwa kwensimu.

Okubi: Izindlela ze-Colorimetric zingaba ngokuqondile futhi zingatholi zonke izinhlobo ze-phenol.

I-5. Imvelo Aboard

I-Biological Asassysed activitions ekhethekile yemithambo yezidalwa zokuthola ubukhona, izakhiwo, nokuqukethwe kwezinto eziqondiswe kuzo. Isibonelo, amanye amagciwane kanye nemvubelo angaguqula i-phenol ibe ngumkhiqizo onemibala ongalinganiswa ngokubuka izinto ezibonakalayo. Lezi zindlela ziqondile kakhulu kepha zingahle zingabi nokuzwela ekugxileni okuphansi.

Izinzuzo: Izindlela zokuzalwa zemvelo ziqondile kakhulu futhi zingasetshenziselwa ukukhomba amakhompiyutha enoveli.

Okubi: Izindlela zokuzalwa zemvelo zingahle zingabi nokuzwela futhi zivame ukudla isikhathi.